oligonucleotide-directed mutagenesis

Site directed mutagenesis (SDM) is a powerful technique where site specific changes in DNA sequence are produced in vitro - for instance to change an amino acid residue into another by changing the codon sequence within the gene sequence.

SDM is also used to 'engineer' commercially important proteins for many different purposes, for example 1. improve stability 2. change specificity 3. reduce toxicity