Making DNA Fingerprints

1) Isolation of DNA.
small amount of tissue, like blood, hair, or skin

2) Cutting
restriction enzymes
e.g. EcoR1, found in bacteria


The DNA pieces are sorted according to size by a sieving technique called electrophoresis.

3) Transfer of DNA to nylon membrance/nitrocellulose membrance

4) Probing.


Uses of DNA Fingerprints

1. Diagnosis of inherited disorders
2. Forensic or criminal
3. Personal identification